Progesterone Testing In Dogs: FAQ for Breeding and Reproductive Health

We've compiled a list of frequently asked questions regarding progesterone testing in dogs, should you have any additional questions or wish to book an appointment with us please call (920) 269-4072, we look forward to speaking with you.  

What is Progesterone Testing in Dogs?

Progesterone testing in dogs measures levels of the hormone progesterone to assess reproductive status, especially for female dogs in breeding programs. Rising progesterone levels indicate ovulation, while dropping levels signal the end of pregnancy or pseudopregnancy (false pregnancy). This information is critical for managing a breeding dog's reproductive cycle.

When Should Progesterone Testing Be Done?

Progesterone testing is essential for timing ovulation and predicting the best time for breeding. Typically, the test is done every 24 to 72 hours when monitoring ovulation. While LH (Luteinizing Hormone) directly triggers ovulation, progesterone rises afterward, making it a more practical hormone to track. Regular testing helps monitor the health of the pregnancy and determine the readiness for labor.

How is Progesterone Testing Performed?

Progesterone testing involves a simple blood test. Some veterinary clinics can run these tests in-house, while others send the samples to a reference lab. The key is having accurate, consistent results, as different clinics may use different machines for progesterone testing.

Why Do Dogs Need Progesterone Testing?

Progesterone testing is primarily used to determine:

  • If a female dog is in heat
  • When she is ovulating
  • When labor is expected to start

This information is vital for ensuring a healthy and successful breeding process.

What Are Normal Progesterone Levels in Dogs?

The interpretation of progesterone levels depends on the purpose of the test. For example, normal levels during ovulation will differ from levels expected at the onset of labor.

How Often Should Progesterone Testing Be Done During a Dog’s Heat Cycle?

The frequency of progesterone testing depends on its purpose:

  • For monitoring ovulation: Every 48 to 72 hours.
  • For confirming pregnancy with an ultrasound: Once around 30 days post-breeding.
  • For monitoring labor: Every 24 hours toward the end of pregnancy.

What Are the Signs a Dog Needs Progesterone Testing?

Progesterone testing is recommended only for female dogs actively involved in a breeding program. It's an essential tool for breeders to optimize the timing of breeding and predict labor accurately.

Can Progesterone Testing Help Determine the Best Time for Breeding?

Yes, progesterone levels are highly predictive of ovulation, which helps pinpoint the best time for breeding. However, variations in test results can occur depending on the machine used for testing.

What Do Low Progesterone Levels in Dogs Indicate?

Low progesterone at the start of heat indicates the dog hasn’t ovulated yet. During pregnancy, low levels may signal a risk of preterm labor, false pregnancy, or the termination of pregnancy.

How Accurate is Progesterone Testing for Predicting Ovulation?

Progesterone testing is considered highly accurate for predicting ovulation, provided that the same testing equipment is used throughout a dog’s reproductive cycle. Consistency in testing methods helps ensure reliable results.

Can Progesterone Testing Diagnose Reproductive Disorders in Dogs?

Yes, progesterone testing can predict preterm labor and help diagnose reproductive issues such as split heats, silent heats, and hormonal imbalances, which may affect fertility.

Are There Any Risks with Progesterone Testing?

The risks associated with progesterone testing are minimal, mainly limited to the blood draw itself, which is a routine veterinary procedure.

How Long Does It Take to Get Progesterone Test Results?

For in-house testing, results can be available in under an hour. However, if the sample is sent to an external lab, results may take anywhere from a few hours to several days.

Can Progesterone Testing Help Diagnose Pregnancy in Dogs?

No, progesterone levels rise at ovulation and stay elevated for about 60 days, regardless of whether the dog is pregnant or not. Other diagnostic tools, such as ultrasound, are needed to confirm pregnancy.

What Age Should a Dog Begin Progesterone Testing?

Progesterone testing is typically used for breeding dogs aged 2 to 8 years, which is considered the prime breeding age range.

Can Progesterone Testing Help Manage Infertility in Dogs?

Yes, progesterone testing can help manage infertility issues by diagnosing conditions such as split heats, silent heats, and hypoluteiodism, where progesterone drops prematurely during pregnancy.

What Are the Different Methods of Progesterone Testing?

Most modern progesterone testing machines use immunoassay technology, which is widely accepted for its accuracy. Older methods like radioimmunoassay are no longer used due to safety and technological advancements.

Are There Alternative Methods for Predicting Ovulation?

While progesterone testing is the most common method, different machines may use varying reagents or techniques. However, alternative tests such as LH testing are not as widely used due to higher costs and practical challenges.

How Much Does Progesterone Testing Cost?

The cost of progesterone testing can range from $100 to $250, depending on factors like whether the test is done in-house or sent to a lab, and additional fees for blood draws and other breeding-related services.

Schedule Progesterone Testing for Your Breeding Dog

Progesterone testing is a crucial tool for any dog breeder looking to maximize reproductive success. Contact us today to learn more about progesterone testing or to schedule an appointment. Our experienced team is here to help you every step of the way in managing your dog’s reproductive health.

Call us at (920) 269-4072 to book an appointment!